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Sequenced positive and negative RNA controls and RNA control mixes collected from various organisms. Products include mRNA, sgRNA, tracrRNA, and crRNA and are ideal for use in gene expression experiments.
5’ppp-dsRNA Control is a negative control for 5’ triphosphate double-stranded RNA (5’ppp-dsRNA), a synthetic ligand for the retinoic acid-inducible protein I (RIG-I, also known as Ddx58). RIG-I is a cytoplasmic RNA helicase that belongs to the RIG-I-like receptors (RLRs) family and triggers an antiviral immune response by the activation of type-I interferons (IFN-alpha and -beta).
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Enhanced green fluorescent protein (EGFP) is a commonly used reporter molecule that emits intense and bright green fluorescence upon excitation EZ CapTM EGFP mRNA (m1 ) is provided at a concentration of approximately 1 mg/mL It features a Cap 1 structure generated through co-transcriptional capping which is more suitable for mammalian systems and offers higher transcription efficiency compared to Cap 0 structures (ARCA and mCap) Incorporation of N1-Methylpseudo-UTP (m1 ) and a poly (A) tail helps suppress RNA-mediated innate immune activation and enhances mRNA stability and lifespan both in vitro and in vivo Additionally the poly (A) tail plays a crucial role in enhancing the efficiency of translation initiation
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Recombinant human erythropoietin (EPO) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of ~18 kDa. This protein is manufactured in human cells using an all-human production system with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding and often supports better stability of the protein in culture. The protein is produced with no artificial tags.
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The SygRNA(R) synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA(R) synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines Cas9 protein or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection lipofection and electroporation.
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